andulex europeaus 1 lectin  (Vector Laboratories)

Journal: BMC Geriatrics

Article Title: Skeletal muscle morphology in sarcopenia defined using the EWGSOP criteria: findings from the Hertfordshire Sarcopenia Study (HSS)

doi: 10.1186/s12877-015-0171-4

Figure Lengend Snippet: A serial cross section showing capillary staining. Capillaries are stained brown and are located at the peripheries of the myocyte. (Capillaries have been stained with Ulex Europeaus Agglutinin 1, 1:200, Vector laboratories, UK, visualised at magnification × 40 and are arrowed)

Article Snippet: Capillaries were stained by incubating separate slides with biotinylated Lectin Ulex Europeaus Agglutinin 1 (UEA-1, Vector Laboratories, Peterborough, UK) at a dilution of 1:200 for two hours (Fig. ).

Techniques: Staining, Plasmid Preparation

Journal:

Article Title: Release of proinflammatory mediators and expression of proinflammatory adhesion molecules by endothelial progenitor cells

doi: 10.1152/ajpheart.00665.2008

Figure Lengend Snippet: A: phase-contrast and fluorescent images of a cord blood-derived “late” endothelial progenitor cell (EPC) colony. Endothelial cells derived from the long-term culture of mononuclear cells (at least 2–4 wk) can form highly proliferative colonies derived from single circulating endothelial cells. Left: phase-contrast image of an EPC colony derived from umbilical cord blood mononuclear cells. Right: confocal microscopy image of an endothelial colony stained with the endothelial surface stain Ulex europaeus lectin (green = fluorescein isothiocyanate label), the nucleus stain 4′,6-diamidino-2-phenylindole (blue) as well as uptake of acetylated low density lipoprotein (red = DiI label). B and C: flow cytometry phenotyping of EPCs using the endothelial surface marker CD31 [platelet endothelial cell adhesion molecule (PECAM)] and the myeloid surface marker CD45. Late EPCs (or colony-forming EPCs) derived from the long-term culture (at least 3–4 wk) of circulating mononuclear cells from adult blood and umbilical cord blood are positive for the endothelial marker CD31 (PECAM) but not for the myeloid marker CD45 while “early” EPCs [or cultured carcinogenic cells (CACs)] derived from the short-term culture (4 days) of adult mononuclear cells also express the myeloid marker CD45. The negative isotype control antibody staining is shown in gray.

Article Snippet: To confirm the endothelial phenotype, adherent cells were incubated with DiI-labeled acetylated low density lipoprotein (LDL; Molecular Probes-Invitrogen, Carlsbad, CA) for 4 h, and, after fixation, they were incubated with the fluorescein isothiocyanate-labeled endothelial surface stain Ulex europeaus lectin 1 (Biomeda, Foster City, CA) for 1 h, as well as the nuclear 4′,6-diamidino-2-phenylindole stain (Molecular Probes-Invitrogen) for 15 min ( 25 ).

Techniques: Derivative Assay, Confocal Microscopy, Staining, Flow Cytometry, Marker, Cell Culture